Graph Splitting (GS) is a brand-new phylogenetic analysis method, which can effectively resolve early evolution of protein families. Its accuracy and speed was proved by extensive evolutionary simulation, and its application to TIM-barrel superfamily highlighted instant evolution of protein-mediated pyrimidine biosynthesis at a transitional phase between the RNA world and the modern DNA-RNA-protein world.

The detailed introduction is available in About GS

To take a glance, please look a sample result (TIM barrels).

Reference: Matsui and Iwasaki., submitted.


Enter protein sequences in multiple-FASTA format:

Note that the sequence number and length should be smaller than 1000 and 2000 aa, respectively.

Or upload a FASTA file:

no file selected

R library (tar ball package, CRAN)

Perl module (tar ball package, CPAN)